Tehran University of Medical Sciences
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Mohammad Hossein Nekoofar

Microleakage of human saliva in coronally unsealed obturated root canals in anaerobic conditions

Authors: Yazdi, K. A. - Bayat-Movahed, S. - Aligholi, M. - Hayes, S. J. - Nekoofar, M. H. -
Keywords: Bacteria, Anaerobic/classification/*isolation & purification , Bismuth/therapeutic use , DNA, Bacterial/analysis , Dental Leakage/classification/*microbiology , Dental Pulp Cavity/*microbiology , Drug Combinations , Electrophoresis, Agar Gel , Epoxy Resins/therapeutic use , Gutta-Percha/therapeutic use , Humans , Polymerase Chain Reaction , Root Canal Filling Materials/therapeutic use , Root Canal Irrigants/therapeutic use , Root Canal Obturation/instrumentation/*methods , Root Canal Preparation/instrumentation/methods , Saliva/*microbiology , Silver/therapeutic use , Sodium Hypochlorite/therapeutic use , Temperature , Time Factors , Titanium/therapeutic use , Tooth Apex/microbiology ,
J Calif Dent Assoc, Vol.37, No.1, 2009,Page:33-7
The purpose of this study was to determine the time required for anaerobic bacteria in natural human saliva to contaminate root-filled teeth. Thirty-two single-rooted teeth were cleaned, shaped, filled, and exposed to human saliva for 120 days. Teeth that had not leaked were subjected to polymerise chain reaction examination. Sixty-six percent of the experimental group were totally contaminated. A PCR examination revealed there was no contamination in the apical 3 mm of leakage-free teeth.