This report covers optimization of radioiodination of peptides by both a direct method in which a constituent tyrosine residue is labeled and indirect method by using an iodinated derivative (SIB) of N succinimidyl 3-(tri-n-butylstannyl) benzoate (ATE) as the intermediate. Radioiodination of IgG and FMLF were performed by direct method using Chloramine-T as an oxidant but since Formyl-Methyl-Leucyl-Phenylalanine, FMLF, does not lend itself for direct radioiodination we performed labeling of FMLF by indirect method via radioiodined SIB at different pH.
Tehran University of Medical Sciences
Synthesis of radioiodinated labeled peptides
Authors: Matloobi M, Rafii H, Beiki D, Khalaj A, Kamali-Dehghan M,
J Radioanal Nucl Chem , Vol.257, No.1, 2003,Page:71-73