Tehran University of Medical Sciences
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Davood Beiki

Increased selectivity in inflammatory site identification via labelling of IgG with N-succinimidyl-4-[125I]iodobenzoate


Authors: Beiki D, Shahhosseini S, Khalaj A, Eftekhari M,
J Label Compd Radiopharm , Vol.45, No.11, 2002,Page:927-934

Human nonspecific polyclonal IgG was labelled with 125I through direct and indirect labelling methods using chloramine-T and a nonphenolic radioiodinated intermediate N-succinimidyl-4-[125I]iodobenzoate (125I-SIB), respectively. Tissue distribution of radioiodinated IgG was assessed in normal and induced inflammation mice. Although, radioiodinated IgG accumulated in the inflammatory area, results showed decreased thyroid and stomach activity and improved inflammatory thigh-to-normal tissue ratios with the indirect labelling method (125I-IB-IgG) compared with the direct labelling method (125I-IgG), indicating reduced in vivo deiodination. These results indicate that the 125I-SIB is probably a preferable approach for labelling antibodies with iodine radioisotopes.