Tehran University of Medical Sciences
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Davood Beiki

High performance liquid chromatographic method for determination of dipyridamole in human plasma


Authors: Beiki D, Pirali M, Amini M, Shafiee A,
DARU, Vol.7, No.2, 1999,Page:14-17

A simple, rapid and specific high-performance liquid chromatographic procedure is reported forquantitative determination of dipyridamole in human -plasma. The assay uses a reversed-phasehigh-performance liquid chromatographic (HPLC) and UV detection at 280nm and has a limitof detection of approximately 5ng/mL. The mobile phase consists of MeOH-H20 (60:40)adjusted to pH 3.3. Dipyridamole was extracted from plasma by back-extraction procedure, withpropranolol as the internal standard. The reproducibility of the method is satisfactory.